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High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle
HRM analysis PCR-RFLP K232A polymorphism
2015/6/9
PCR-RFLP analysis is a common method for genotyping the DGAT1 K232A polymorphism in cattle. Our purpose was to develop a high resolution melting (HRM) assay in order to genotype the polymorphic allele...
Mutation in the RPE65 gene causing hereditary retinal dystrophy in the Briard dogs: application of a new detection method
RPE65 gene CSNB dog Briard electrophoresis
2015/6/5
Inherited eye diseases are widespread in most of the pure dog breeds and they show a severe impact on canine health, welfare and working ability. Congenital stationary night blindness (CSNB) was origi...
Determination of carcinoembryonic antigen and cancer antigen values with the radioimmunoassay method in healthy females dogs
canine mammary gland CEA CA 15-3 tumor markers
2015/4/17
The aim of this study was to determine reference values of carcinoembryonic antigen and cancer antigen in 32 clinically healthy bitches. The average age of the bitches in each group was as follows: sm...
Validation of a simple method for the interpretation of uterine cytology in cows
subclinical endometritis dairy cattle polymorphonuclear neutrophils ROC curves
2015/4/16
One of the main drawbacks of using endometrial cytology in cows is the time required for sample collection and interpretation. It is recommended to count a large number of polymorphonuclear neutrophil...
Application of an electrometric method for measurement of in vitroinhibition of blood cholinesterases from sheep, goats and cattle by dichlorvos and carbaryl
cholinesterase organophosphate carbamate electrometric method in vitro ruminant
2015/3/5
A modified electrometric method was described in sheep, goats and cattle and used to demonstrate in vitro inhibition of plasma and erythrocyte cholinesterase activities by the organophosphate and carb...
Counter immunoelectrophoresis: a simple method for the detection of species-specific muscle proteins in heat-processed products
counter-immunoelectrophoresis muscle protein thermostable antigen antiserum heat-processed meat products
2015/1/26
Counter immunoelectrophoresis (CIE) was used for the detection of species-specific muscle proteins in food products. This technique allowed the detection of pork, beef, poultry, or and kangaroo meats ...
A Simple Method for Sexing Chicken Embryos at Stage X
blastodermal cell chicken embryo embryo culture PCR sexing
2009/7/31
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by...
Preparation of Monoclonal Antibody Against Chicken Apolipoprotein B and Development of Enzyme Liked Immunosolvent Assay (ELISA) Method with the Antibody Aiming at the Optimization of Lipid Metabolism in Chickens
apolipoprotein B monoclonal antibody enzyme linked immunosorbent assay fat deposition broiler chickens
2009/7/31
Apolipoprotein B (apoB) containing lipoproteins are major lipoproteins in blood and the elevated concentration in plasma may lead to increased transport of lipoproteins into adipose tissues resulting ...
A Fermentation Method to Dry and Convert Shochu Distillery By-product to a Source of Protein and Enzymes
Aspergillus Awamori feed shochu distillery by-product
2009/7/31
Shochu distillery by-product (SDBP) is usually dried before using as an ingredient of formula feed. But drying is costly and causes destruction of the nutrients. In the present study, a new technique ...
The Cloacal Test:a Method for Testing Anti-androgenic Effects of Chemicals in Birds
anti-androgenic chemical cloacal protrusion androgen Japanese quail
2009/7/31
The aim of this study was to develop a method to test the anti-androgenic effects of chemicals in birds. Male Japanese quail were castrated to lead the regression of cloacal protrusion, and they were ...
Determination of Immunoglobulin Y Concentration in Yolk Extract Prepared by Water Dilution Method:Comparisons among Three Strains of Chickens
chicken egg yolk IgY concentration plasma water dilution method
2009/7/29
In avian species, immunoglobulin Y (IgY) is found abundantly in egg yolk as well as in blood. Although the concentration of IgY in yolk has been previously reported by many investigators, the reported...
Working Memory Learning Method and Astrocytes Number in Different Subfields of Rat's Hippocampus
Hippocampus astrocytes working memory spatial learning
2009/1/19
The aim of this study was evaluation of the astrocytes number in different subfields of rat's Hippocampus after spatial learning with usage of Morris Water Maze technique and working memory method. I...