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A simple method for assessing hyaluronic acid production by cumulus-oocyte complexes
oocyte meiotic maturation cumulus expansion
2016/7/6
The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such it is a possible biomarker of the in vitro maturing oocytes quality. Cumulus e...
Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems
embryo transfer MOET oviduct uterus superovulated sheep
2015/6/5
The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and sevent...
Use of the Non-electrophoretic Method to Detect Testis Specific Protein Gene for Sexing in Preimplantation Bovine Embryos
Bovine Embryo Sex Determination TSPY Gene PCR
2016/4/25
Testis-specific protein (TSPY) is a Y-specific gene, with up to 200 copy numbers in bulls. In order to make bovine embryo sexing under farm condition more feasible, the possibility of using a non-elec...
Variance Component Estimates with Dominance Models for Milk Production in Holsteins of Japan Using Method R
Method R Additive Variance Dominance Variance Sire by Herd Interaction Inbreeding Depression Milk Production, Holstein
2016/4/21
Fractions of herd-year-season, sire by herd interaction, additive genetic and dominance genetic variances were estimated for milk production traits in Holsteins of Japan using Method R. Inbreeding dep...
Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages: a review
somatic cloning microsurgical nuclear transfer karyoplast intraooplasmic injection chromatin remodelling epigenetic reprogramming
2015/6/1
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alte...