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基于高通量测序的大麦InDel标记开发及应用
大麦 重测序 InDel标记 品种鉴定
2020/12/31
分子标记是遗传研究的基础工具, 广泛应用于遗传多样性研究、种质鉴定、遗传图谱构建和基因定位等领域。本研究基于大麦的全基因组重测序数据鉴定出的二态性SNP位点, 开发出遍布全基因组的118对InDel引物。以49份不同地理来源的大麦种质检测其有效性, 筛选出2个等位基因的共显性InDel标记72对, 进一步对288份大麦种质进行遗传距离分析及构建系统发育树。结果显示, 筛选到32个有效性的核心InD...
InDel Marker Based Estimation of Multi-Gene Allele Contribution and Genetic Variations for Grain Size and Weight in Rice (Oryza sativa L.)
Insertion/Deletion (InDel) markers genetic variation multi-gene allele contributions rice (Oryza sativa L.), grain size and weight rice germplasm
2023/8/9
The market success of any rice cultivar is exceedingly dependent on its grain appearance, as well as its grain yield, which define its demand by consumers as well as growers. The present study was und...
西瓜枯萎病生理小种1抗性QTL精细定位与InDel标记开发
西瓜 枯萎病生理小种1 QTL精细定位 InDel标记
2017/11/24
通过QTL初定位检测栽培西瓜枯萎病生理小种1抗性的主效QTL，验证枯萎病抗性基因Fon-1的存在，结合亲本重测序信息开发紧密连锁易于检测的InDel (insertion/deletion)分子标记，为西瓜枯萎病分子标记辅助育种提供技术支撑，并实现该QTL的精细定位，加速Fon-1的克隆和功能验证进程。【方法】以高抗枯萎病的栽培西瓜‘ZXG01478’和高感病的栽培西瓜‘14CB11’为亲本构建的...
Genetic Relationships of Sorghum Germplasm in Asia and Africa Revealed by Rice cDNA-STS and Indel Markers
genetic diversity grain sorghum
2010/10/25
The elucidation of Sorghum bicolor intraspecific genetic diversity is of interest in studies on evolutionary forces under domestication and geographical distribution, and has application in the design...