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Improvement of the Thermal Stability of a Calcium-free, Alkaline α-Amylase by Site-directed Mutagenesis
Bacillus alkaliphile α-amylase thermostability site-directed mutagenesis
2008/4/20
Alkaline α-amylase from Bacillus sp. strain KSM-K38 (AmyK38) is a calcium-free enzyme that is stable against chelating and oxidative reagents. Recently, the thermostability of this enzyme was improved...
Enhancing the Thermal Stability of Sucrose Phosphorylase from Streptococcus mutans by Random Mutagenesis
sucrose phosphorylase Streptococcus mutans random mutagenesis thermostable enzyme
2008/4/20
The thermal stability of sucrose phosphorylase (EC 2.4.1.7) from Streptococcus mutans was enhanced using random and site-directed mutageneses. Random mutagenesis studies revealed that eight single ami...