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Development and application of LC/HRPS for quantification of adenine nucleotides,creatine phosphate,and creatine in sturgeon spermatozoa
macroergic phosphates high resolution mass spectrometry extraction method validation sperm chondrostean fishes
2017/2/21
The objective of this study was to investigate the applicability of liquid chromatography coupled with high resolution product scan (LC/HRPS) mass spectrometry for simultaneous quantification of adeni...
Effect of the application of bioplexes of zinc, copper and manganese on milk quality and composition of milk and colostrum and some indices of the blood metabolic profile of cows
dairy cows bioplexes of zinc copper and manganese colostrum milk blood
2015/6/4
The object of an experiment was inorganic and organic forms of zinc, copper and manganese applied in mineral mixtures to dairy cows. The experiment was carried out on 90 cows with average milk yield f...
Effect of Exogenous Fibrolytic Enzyme Application on the Microbial Attachment and Digestion of Barley Straw In vitro
Exogenous Feed Enzymes, Rumen Bacteria, Straw, Ammoniation, In vitro
2016/5/9
The effects of exogenous fibrolytic enzymes (EFE; a mixture of two preparations from Trichoderma spp., with predominant xylanase and β-glucanase activities, respectively) on colonization and digestion...
Development and Application of High-density SNP Arrays in Genomic Studies of Domestic Animals
Whole-genome Sequencing HapMap SNP Array Genomics Domestic Animals
2016/5/5
In the past decade, there have been many advances in whole-genome sequencing in domestic animals, as well as the development of “next-generation” sequencing technologies and high-throughput genotyping...
Myogenic Satellite Cells and Its Application in Animals - A Review
Satellite Cells Myogenesis Plasticity Transdifferentiation Adipogenesis Review
2016/5/3
Myogenic satellite cells have been isolated and identified by several recently elucidated molecular markers. Furthermore, knowledge about the precise function of these markers has provided insight int...
双壳纲动物核糖体RNA 18S-ITS1序列及其在分子系统发育研究中的应用18S-ITS1 Sequence of rRNA in Bivalves and its Application in Phylogenetic Analysis
ITS1 18S RNA 遗传多样性 双壳纲
2008/1/20
摘要
测定了双壳纲不同科、属、种及种内共11个个体的核糖体RNA 18S-ITS1序列。结果表明，该序列在种间存在很高的多态性，长度从558 bp到784 bp不等，碱基差异百分比在10.7%~61.7%之间， ITS1序列同源性很低，有片段的插入与缺失。种间18S部分序列碱基差异百分比在0.9%~23.7%之间，变化主要是碱基的转换。用邻接法（NJ）构建了8个种的18S部分序列（约240 bp...
GFP标记在转基因青鳉同系繁殖纯化中的应用Application of GFP Mark on Inbred Depuration of Transgenic Medaka (Oryzias latipes)
绿色荧光蛋白（GFP）基因 显微注射 青鳉 同系繁殖 纯化
2007/12/31
摘要从载体pBluescript SK+的Nde I位点处插入外源基因片段，构建了含有青鳉（Oryzias latipes）β-肌动蛋白（β-Actin）启动子和绿色荧光蛋白（GFP）标记基因的新表达载体。显微注射实验证实：在青鳉受精卵的单细胞期进行显微注射，标记基因的表达率和胚胎的存活率均较高。对嵌合体的转基因青鳉（F1）进行了两代的杂合体（F2和F3）筛选和一代的纯合体（F4）筛选，获得第4代...
应用双色荧光原位杂交技术检测克氏综合征The Application of Dual-color Fluorescence in situ Hybridization to the Diagnosis of Klinefelter Syndrome
荧光原位杂交 克氏综合征 性染色体异常 DNA特异性探针
2007/12/30
摘要正常对照标准值4.17%）。用FISH 技术检测性染色体数目异常克氏综合征具有快速、敏感度高、信号强、背景低、多色等优点，故FISH 技术在产前诊断检测领域中显示其重要的应用价值和发展前景。
Abstract:The objective of the work is to study the technique of dual-color fluorescence in situ hybri...
Microencapsulation of Korean Mistletoe (Viscum album var. coloratum) Extract and Its Application into Milk
Microencapsulation Korean Mistletoe Extract Polyglycerol Monostearate Medium-chain Triacylglycerol Milk
2016/4/27
This study was designed to develop microencapsulated Korean mistletoe extract, to determine the stability in vitro and to examine its application in milk. Coating materials used were polyglycerol mono...
Application of Recent DNA/RNA-based Techniques in Rumen Ecology
Rumen Microbial Ecology, Ribosomal Molecular Functional Analysis
2016/4/25
Conventional culture-based methods of enumerating rumen microorganisms (bacteria, archaea, protozoa, and fungi) are being rapidly replaced by nucleic acid-based techniques which can be used to charact...
Analysis of Microsatellite DNA Polymorphisms in Five China Native Cattle Breeds and Application to Population Genetics Studies
China Native Cattle Genetic Diversity Microsatellite Markers Population Genetics
2016/4/20
Five China native cattle breeds have been characterized by using 10 microsatellite DNA markers. The studied populations can be divided into five groups: Luxi cattle, Nanyang cattle, Jinnan cattle, Qin...
Application of Gel-based Proteome Analysis Techniques to Studying Post-mortem Proteolysis in Meat
Proteolysis Proteome Analysis Skeletal Muscle
2016/4/18
This study was conducted to evaluate the possible application of 2 D-SDS-PAGE (2 DE)-based proteome analysis techniques to the assessment of extreme proteolysis in postmortem skeletal muscle. Eight Ha...
Deodorization of Pig Feces by Fungal Application
Candida rugosa Candida maris Volatile Fatty Acids Pig Feces
2016/4/18
This study was carried out to screen yeasts effective in reducing odor from pig feces. Three isolates from soil and compost sources were selected to treat pig feces. On the basis of morphological and ...
Isolation of Bacillus SP. as a Volatile Sulfur-Degrading Bacterium and Its Application to Reduce the Fecal Odor of Pig
Bacillus SP. Comamonas Testosteroni Hydrogen Sulfide Malodor Pig Feces
2016/4/14
Fecal malodor is an acute environmental issue to be solved for the intensive animal agriculture in Japan. Among these substances volatile sulfur such as hydrogen sulfide (HS), methanethiol, and dimeth...
Application of Gene Transfer Technology for Genetic Improvement of Fish
Transgenic fish genetic improvement of fish gene transfer aquaculture
2010/3/29
The worldwide decline of natural fish stocks, the accumulation of chemical pollutants in aquatic environments and increasing world consumption of fish products has provided an impetus in many countrie...