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利用SDS-PAGE 分析、PCR 扩增和序列测定与分析研究了长发带芒草(Taeniatherumcrinitum) 的高分子量谷蛋白亚基及其基因。结果显示, 长发带芒草中发现的高分子量谷蛋白亚基与普通小麦中的类似, 但迁移率存在较大差异。其中, x 型亚基均比Dx2 亚基迁移率小或接近, y 型亚基均比Dx12 亚基迁移率更快。本研究结果揭示了带芒草属具有与普通小麦类似的高分子量谷蛋白亚基, 这...
微卫星PCR产物变性与非变性PAGE-银染检测方法的比较The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites
微卫星 PAGE PCR 银染
2008/1/8
摘要
用鸡的微卫星引物对6个中国地方鸡种的两个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶（PAGE）上进行电泳，经银染，表明微卫星产物在二者上的电泳结果有明显的差异。在非变性聚丙烯酰胺凝胶中，表现为有较多的非特异带，而在变性胶中微卫星扩增产物条带清晰，易于鉴定。Abstract：The genomic DNAs from six chicken breeds in Chin...
Comparison of Skeletal Muscle Protein Bands among Five Populations of Bufo viridis in Turkey by SDS-PAGE
Skeletal muscle SDS-PAGE Bufo viridis protein band
2010/3/26
Skeletal muscle protein bands of Bufo viridis samples from 5 different populations in Turkey were investigated by SDS-PAGE (Sodium Dodecyl Sulphate-Poly Acrylamide Gel Electrophoresis). The total numb...
SDS-PAGE Patterns of Blood Serum Proteins in some Species of the Genus Meriones (Mammalia: Rodentia)
SDS-PAGE Serum Meriones Turkey
2010/3/30
The blood serum proteins of Meriones meridianus, Meriones crassus, Meriones persicus and Meriones tristrami, all found in Turkey, along with domestic Meriones unguiculatus, which originated in Mongoli...