搜索结果: 1-5 共查到“quantitative PCR”相关记录5条 . 查询时间(0.078 秒)
Semi-Quantitative PCR for Quantification of Hepatotoxic Cyanobacteria
Cyanobacteria Microcystis Competitive PCR mcyB cpcBA
2013/3/11
Blooms of microcystin-producing cyanobacteria are a problem worldwide. Microcystin is a liver hepatotoxin commonly found in bodies of water and is produced mainly by the genus Microcystis. The aim of ...
［目的］为鹦鹉热衣原体的快速、准确检测奠定基础。［方法］根据鹦鹉热衣原体主要外膜蛋白(MOMP)基因序列设计一对特异性引物，以SYBR GreenⅠ为荧光染料，建立了鹦鹉热衣原体Realtime quantitative PCR检测方法。根据检测结果，计算样品的批内和批间变异系数（CV）。［结果］以提取的鹦鹉热衣原体DNA为模板，用引物Cps1和Cps2进行PCR扩增，得到长100 bp...
Detection of Hemizygous Chromosomal Copy Number Variants in Williams-Beuren Syndrome (WBS) by Duplex Quantitative PCR Array: An Unusual Type of WBS Genetic Defect
human chromosome 7 deletion copy number variants genome
2009/4/14
We have developed a dual probe quantitative PCR (qPCR) mini array enabling a more accurate analysis of the relationship between copy number variants (CNVs) and other genomic features in specific areas...
新定量PCR数据处理方法的理论探讨A New Method of Processing Quantitative PCR Data
定量PCR PCR动力学 动力学数学模型 荧光定量PCR
2007/12/30
摘要日新月异的生命科学技术的发展及临床医学科学研究的需求，一般的PCR技术已远远不能满足工作的需要。PE公司在进行了大量的PCR动力学研究的基础上，发现了利用荧光标记探针在PCR循环过程中积累的荧光强度达到仪器捡出阈值时，系统的初始模板数量与循环次数之间有线性关系，据此建立了目前的PE 7700 、PE 5700仪器的定量PCR技术,开创了PCR技术的新局面。但是由于这一技术的误差较大，尚不能满足...
Wide-ranging abundances of aerobic anoxygenic phototrophic bacteria in the world ocean revealed by epifluorescence microscopy and quantitative PCR
aerobic anoxygenic phototrophic bacteria world ocean revealed epifluorescence microscopy quantitative PCR
2014/5/9
Reports that aerobic anoxygenic phototrophic bacteria (AAnPB) containing bacteriochlorophyll a (BChla) constitute 10% or more of total bacteria cell counts in seawater prompted us to quantify AAnPB ab...