搜索结果: 1-10 共查到“RNA Interference”相关记录10条 . 查询时间(0.177 秒)
Development of marker-free rice with stable and high resistance to rice black-streaked dwarf virus disease through RNA interference
marker-free rice black-streaked dwarf virus disease RNA
2023/8/9
The rice black-streaked dwarf virus (RBSDV) disease causes severe rice yield losses in Asia. RNA interference (RNAi) has been widely applied to develop antiviral varieties in plants. So far, only a fe...
SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells
SARS-CoV-2-encoded nucleocapsid protein acts viral suppressor of RNA interference cells
2020/6/12
We thank Prof. Peng Zhou (Wuhan, China) for kindly providing materials and thank Yan Wu and Weijuan Shang for experimental assistance. This work was supported by the Strategic Priority Research Progra...
Fundamental study of detection of muscle hypertrophy-oriented gene doping by myostatin knock down using RNA interference
In vitro and in vivo transformation C2C12 cultured cell
2015/5/11
To investigate the feasibility of developing a method for detection of gene doping in power-athletes, we devised an experimental model system.
Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2016/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Targeted Suppression of Connexin 43 in Ovine Preimplantation Embryos by RNA Interference Using Long Double-stranded RNA
Connexin 43 RNA Interference Ovine Embryo
2016/5/4
RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of...

Ever since RNA interference was discovered, in 1998, scientists have been pursuing the tantalizing ability to shut off any gene in the body — in particular, malfunctioning genes that cause diseases su...
RNA Interference as a New Tool in Therapeutics
RNA interference small interfering RNA off-target effects
2010/2/1
The basic principle of RNA interference, a possible new therapeutic tool, involves destruction of messenger RNA upon interaction with homologous double-stranded RNA present in the cell cytoplasm. Stud...
Use of RNA interference technology for cancer specific gene silencing
RNA interference siRNA cancer gene therapy
2009/7/15
RNA interference (RNAi) can be applied to human cancer cells by using small interfering RNAs (siRNAs) for inhibition of activated oncogenes' expression with the amelioration of several malignant pheno...
RNA干涉及其应用前景RNA Interference and Its Promising Future
RNA干涉 小干涉RNA 转录后基因沉默 Dicer酶 RdRP活性 功能基因组
2007/12/30
摘要RNA干涉是指由特定双链RNA(dsRNA)引起的转录后基因沉默现象。研究表明,Dicer断裂dsRNA产生的小干涉RNA可以抑制哺乳动物体细胞和胚胎中的基因的表达。 RdRP在扩增RNAi中起着关键性的作用,RdRP活性复制较长的触发性dsRNA或以一种非引物的方式复制短的siRNA,即以siRNA为引物的RdRP反应使靶mRNA转变为dsRNA,同时复制触发性dsRNA。 所有的产物又可作...
摘要RNAi是多种生物体内由dsRNA介导的同源mRNA降解现象。这是一个高度特异化的过程,涉及多种蛋白质的共同参与。在这一过程中,siRNA的结构影响其两条链装配到RISC中去的能力。除了与RISC结合外,siRNA还引导了RITS复合物结合到同源染色质,介导异染色质化过程。干扰效应的扩散,即系统性沉默可能依赖于跨膜蛋白的转运,并且很可能是在多因素调控下完成的。Abstract: RNA int...