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Identification and isolation of epididymal luminal proteins of the mouse
Identification isolation epididymal luminal proteins mouse
2009/2/23
Proteins of mouse cauda epididymal fluid were analyzed by polyacrylamide gel electrophoresis. Fluid expressed from the cauda epididymidis and samples obtained by micropuncture of the epididymal lume...
Cell- and region-specific localization of lysosomal and secretory proteins and endocytic receptors in epithelial cells of the cauda epididymidis and vas deferens of the adult rat
Cell- and region-specific localization secretory proteins endocytic receptors
2009/2/18
The epithelial cells lining the cauda epididymidis and vas deferens are active in endocytosis and have an abundance of lysosomes and a well-characterized secretory apparatus. However, little is kn...
Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat
Adhesion signaling proteins spermiation rat
2009/2/18
Spermiation, the process by which late spermatids separate from the Sertoli cell, is disrupted by a number of toxicants. In this study, we used immunohistochemistry (IHC) to identify some of the p...
Evidence for the role of heterotrimeric guanine nucleotide-binding regulatory proteins in the regulation of the mouse sperm adenylyl cyclase by the egg's zona pellucida
Pertussis toxin acrosome reaction mastoparan cyclic
2009/2/18
Sperm acrosomal exocytosis is the result of a complex set of signal transduction pathways activated physiologically by the egg's extracellular matrix, the zona pellucida. In the mouse, the zona pe...
Potential involvement of kallikrein hK2 in the hydrolysis of the human seminal vesicle proteins after ejaculation
Seminal plasma semenogelin fibronectin protein C
2009/2/17
We have recently demonstrated in liquefied human seminal plasma the presence of the novel kallikrein hK2 in association with protein C inhibitor (PCI) as a 75-kDa complex. In the present study, we s...
Detection of germ cell-derived proteins in testicular interstitial fluid: potential for monitoring spermatogenesis in vivo
Seminiferous tubule-secreted proteins phosphatidylethanolamine binding protein ARP-2 spermatid
2009/2/16
The aim of the present study was to assess whether proteins secreted by the seminiferous tubules (ST) can be detected in testicular interstitial fluid (IF) and testicular (TV), spermatic (SV), and p...
Two-dimensional electrophoretic profile of human sperm membrane proteins
Human spermatozoa membrane proteins two-dimensional electrophoresis contraceptive vaccine
2009/2/13
The purpose of this study was to characterize highly enriched human spermatozoa membrane proteins by two-dimensional electrophoresis and computer image analysis. Sperm membrane proteins were extract...
150-kDa proteins in dog serum bind 1.5-kDa growth-promoting factors for androgen-independent canine prostatic epithelial cells
150-kDa proteins dog serum bind 1.5-kDa growth-promoting factors androgen-independent
2009/2/12
Fractions obtained by gel filtration or ultrafiltration of dog serum were tested for their mitogenic activity on canine prostatic epithelial cells: two prostatic growth factor (PGF) entities were fo...
Gastricsin-mediated proteolytic degradation of human seminal fluid proteins at pH levels found in the human vagina
Gastricsin-mediated proteolytic degradation human seminal fluid proteins pH levels
2009/2/12
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigated. Upon acidification to the pH level of the human vagina, autoproteolysis of most seminal fluid ...
Sperm surface proteins after capacitation. Expression of vitronectin on the spermatozoan head and laminin on the sperm tail
Sperm surface proteins vitronectin spermatozoan head laminin sperm tail
2009/2/11
Several integrins recognize as ligands proteins containing the Arg-Gly-Asp (RGD) sequence, such as fibronectin, vitronectin, and laminin. It has been previously demonstrated that oligopeptides con...
Characterization of sulfhydryl proteins involved in the maintenance of flagellar straightness in hamster spermatozoa
sperm flagella motility sulfhydryl proteins
2009/2/6
Hamster caput epididymal spermatozoa exhibit a marked 90-180 degree bend when induced to acquire progressive motility in vitro (Cornwall et al, 1988). Flagellar bending is prevented by oxidizing spe...
Proteins in luminal fluid of the ram excurrent ducts：changes in composition and evidence for differential endocytosis
sperm proteins androgen-binding protein endocytosis rete testis fluid ductuli efferentes
2009/2/6
Rete testis fluid (RTF) and luminal fluid collected by micropuncture at selected epididymal sites were analyzed to characterize the spectrum of proteins and to quantify the net gain or loss of total...
Dibromoacetic acid, a prevalent by-product of drinking water disinfection, compromises the synthesis of specific seminiferous tubule proteins following both in vivo and in vitro exposures
Disinfection by-products spermatogenesis proteomics
2008/12/29
Dibromoacetic acid (DBA) is a by-product of drinking water disinfection that alters spermatogenesis in adult male rats. To identify a mechanism by which DBA alters spermatogenesis, seminiferous tubu...
Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath
human sperm fibrous sheath proteins AKAP4 AKAP3 dysplasia
2008/12/26
Dysplasia of the fibrous sheath (DFS) is characterized by male infertility, asthenozoospermia, and morphologically abnormal flagella that possess a severely malformed fibrous sheath. In many cases, DF...
Actin and actin-binding proteins in bovine spermatozoa：potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
proteins bovine spermatozoa potential role signaling epididymal maturation
2008/12/26
The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells, including shape, movement, and interactions with extracellular matrices. The role of actin in mammalian male ger...