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均匀设计优化棉花ISSR-PCR反应体系
棉花 ISSR 体系优化 均匀设计
2013/8/6
为探索适宜棉花的ISSR-PCR反应体系，采用两轮均匀设计试验，对ISSR-PCR反应体系中Mg2+ 、dNTPs、模板DNA、引物、Taq DNA聚合酶的浓度或用量进行优化。结果表明，棉花20 μL的ISSR反应体系的最佳组分包括2 μL 10×PCR Buffer、0.5 U Taq DNA聚合酶、40 ng模板DNA、0.25 mmol·L-1 dNTPs、0.5 μmol·L-1引物和2....
Distinguishing grapefruit and pummelo accessions using ISSR markers
accession Citrus maxima Citrus paradisi germplasm characterization molecular markers
2014/3/10
Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus par...