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Evaluation of Streptomyces as a Probiotic Feed for the Growth of Ornamental Fish Xiphophorus helleri
probiotics Actinobacteria Streptomyces ornamental fish Xiphophorus helleri
2011/2/24
The potential of Streptomyces as a probiotic feed for the growth of ornamental fish Xiphophorus helleri has been investigated. The Streptomyces strains used as probiotics were isolated from the marine...
Regulatory Elements in Tetracycline-Encoding Gene Clusters: the otcG Gene Positively Regulates the Production of Oxytetracycline in Streptomyces rimosus
type II polyketide synthase oxytetracycline biosynthesis Streptomyces rimosus
2010/2/1
The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins that can have either a pathway-specific or a pleiotropic mode...
Aminopeptidases in Mycelium and Growth Medium of Streptomyces rimosus Strains
prolyl leucyl and arginyl aminopeptidases localization
2010/2/1
Aminopeptidases (APs) of the same substrate specificities have been detected in the mycelia and culture filtrate of Streptomyces rimosus. To compare extracellular and intracellular prolyl, leucyl and ...
链霉菌F0107液体发酵产木聚糖酶条件的优化（Optimization of Xylanase Production by Streptomyces F0107 under Submerged Fermentation）
链霉菌 木聚糖酶 筛选 发酵
2009/8/11
运用透明圈法从土样中筛选出产木聚糖酶放线菌80余株。对其中一株产酶情况较稳定的链霉菌菌株F0107进行了液体发酵的碳源、氮源、初始pH值、摇床转速及培养温度等条件的优化。实验表明，此菌株最适碳源为玉米芯水不溶性木聚糖，最适氮源为酵母浸膏与蛋白胨的复合氮源，最适初始pH值为5.0，最适产酶温度和最适摇床转速分别为32℃和140r/min。质量分数为0.4%的吐温80使菌株木聚糖酶产量提高41%，发酵...
Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
arabinan α-L-arabinofuranosidase glycoside hydrolase family 43
2009/6/18
An α-L-arabinofuranosidase gene of Aspergillus oryzae was expressed in Pichia pastoris. The re-combinant enzyme released L-arabinose from arabinose-containing polysaccharides such as lupin pectic gala...
Comparison of Enzymatic Properties of Microbial Transglutaminase from Streptomyces sp.
microbial transglutaminase Streptomyces purification
2009/6/17
The microbial transglutaminase (TGase) from Streptomyces libani was purified from its culture broth and its enzymatic properties were compared with those of TGase from Streptoverticillium mobaraense v...
Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
α-galactosidase Streptomyces coelicolor A3(2) glycoside hydrolase
2009/6/10
An α-galactosidase gene belonging to glycoside hydrolase family 27 from Streptomyces coelicolor was cloned and expressed in E. coli. The purified enzyme showed a single protein band on SDS-PAGE with a...
The in vivo Expression of Streptomyces rimosus tRNA Genes
Streptomyces rimosus expression promoter
2009/3/23
The expression of seven tRNA genes from Streptomyces rimosus, cloned on bifunctional plasmids, has been studied in a homologous (S. rimosus) and a heterologous (Escherichia coli) system. Analyzed gene...
High Efficiency of Direct Plasmid Transfer between Two Streptomyces spp., and between Streptomyces spp. and E. coli
electroduction Streptomyces E. coli
2009/3/20
Electroduction is a method which allows direct transfer of plasmid DNA between donor and recipient under conditions of electroporation. The application of this technique allows significantly faster an...
Ribosomal RNA Operons in Streptomyces rimosus:Sequence of the rrnF and Comparative Analysis
rRNA operon multiple promoters streptomycetes
2009/3/20
One of the six Streptomyces rimosus ribosomal RNA operons, rrnF, was completely sequenced. rRNA genes, arranged in the order 16S-23S-5S, encode 1529, 3121 and 120 nucleotides long rRNAs, respectively....
Streptomyces rimosus GDS（L）Lipase: Production,Heterologous Overexpression and Structure-Stability Relationship
lipase Streptomyces plate assay
2009/3/19
Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time...
Persistence of the Chromosome End Regions at Low Copy Number in Mutant Strains of Streptomyces rimosus and Streptomyces lividans
linear chromosome linear plasmid deleted DNA
2009/3/16
Streptomycetes are important antibiotic producing bacteria that often exhibit genetic instability. One or both ends of the linear Streptomyces chromosome are lost spontaneously, resulting in viable mu...
Influence of Mode of Fermentation on Production of Polygalacturonase by a Novel Strain of Streptomyces lydicus
Streptomyces lydicus polygalacturonase pectin lyase
2009/3/12
Production of different pectinolytic enzymes was attempted using the actinomycete strain Streptomyces lydicus in submerged fermentation. Polygalacturonase and pectin lyase activities were detected in ...
航天诱变提高Streptomyces gilvosporeus的那他霉素产量
space-flight mutation Streptomyces gilvosporeus natamycin rational screening
2009/3/10
Mutants of the strain producing natamycin, Streptomyces gilvosporeus, were obtained after space-flight mutation. With respect to the sand spores and slant spores, the mutation ratios were up to 67.6% ...
Formation of Edible Soybean and Soybean-Complex Protein Films by a Cross-Linking Treatment with a New Streptomyces Transglutaminase
transglutaminase edible films soybean protein isolates whey protein isolates
2009/2/26
Soybean protein isolate (SPI) was used in the investigation of the formation of edible protein films through an enzymatic cross-linking method with a purified microbial transglutaminase (MTG) produced...